Determination of acetylsalicylic acid and related substances in pharmaceutical preparations and bulk drugs by capillary electrophoresis
نویسندگان
چکیده
Micellar electrokinetic chromatography (MEKC) using sodium dodecyl sulphate (SDS) in separation buffer has been employed for the separation and determination of acetylsalicylic acid and related compounds in tablets and bulk drugs. Base line separation was achieved by using 12.5 mM sodiumtetraborate decahydrate, 15 mM boric acid (pH 9.0) and 50 mM SDS. Analytical characteristics such as migration time, relative migration time and relative response factor were determined. Acetaminophen was used as internal standard to correct and compensate errors due to injection and evaporation losses. Drug content in formulations and bulk drugs was determined and the recovery was between 98.6 and 101.5%. All the preparations and bulk drugs examined were found to contain the hydrolyzed and starting material of salicylic acid, in varying amounts.
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